WISP1 Protein (Transcript Variant 1)
This Recombinant WISP1 protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for WISP1 Protein (Transcript Variant 1) (ABIN2735599)
抗原
See all WISP1 蛋白
WISP1
(WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human WISP1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 23-367) protein (His tag)
WISP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 1-280) protein (GST tag)WISP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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别名
- Wisp1
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背景
- This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
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分子量
- 38.1 kDa
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NCBI登录号
- NP_003873
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途径
- WNT signaling, Growth Factor Binding
抗原
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