产品编号 ABIN2728169
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for PAK2 蛋白 (ABIN2728169)
抗原
See all PAK2 蛋白
PAK2
(P21-Activated Kinase 2 (PAK2))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
纯度
> 90 % as determined by SDS-PAGE and Coomassie blue staining
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human PAK2 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Specific activity was determined as 85,806 pmoles/min/μg, according to the Zlyte assay protocol
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P21-Activated Kinase 2 (PAK2) (AA 131-230) protein (GST tag)
PAK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
P21-Activated Kinase 2 (PAK2) (AA 227-524) protein (His-IF2DI Tag)PAK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PAK2 (P21-Activated Kinase 2 (PAK2))
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别名
- Pak2
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背景
- The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
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分子量
- 33.5 kDa
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NCBI登录号
- NP_002568
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途径
- MAPK Pathway, RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
抗原
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