PAK1 Protein (Transcript Variant 1)
Quick Overview for PAK1 Protein (Transcript Variant 1) (ABIN2728167)
抗原
See all PAK1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human PAK1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Specific activity was determined as 32,638 pmoles/min/μg, according to the Zlyte assay protocol
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P21-Activated Kinase 1 (PAK1) (AA 1-447) protein (GST tag)
PAK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
P21-Activated Kinase 1 (PAK1) (AA 1-545), (full length) protein (His-SUMO Tag)PAK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
P21-Activated Kinase 1 (PAK1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PAK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PAK1 (P21-Activated Kinase 1 (PAK1))
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别名
- Pak1
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背景
- This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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分子量
- 33.6 kDa
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NCBI登录号
- NP_001122092
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途径
- MAPK Pathway, RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis
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