MGMT Protein (Myc-DYKDDDDK Tag)
Quick Overview for MGMT Protein (Myc-DYKDDDDK Tag) (ABIN2727841)
抗原
See all MGMT 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This MGMT protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human O-6-methylguanine-DNA methyltransferase (MGMT) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
custom-made MGMT 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-211) protein (His tag)custom-made MGMT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)MGMT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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别名
- O-6-Methylguanine-Dna Methyltransferase (Mgmt)
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背景
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProtKB/Swiss-Prot Function]
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分子量
- 24.9 kDa
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NCBI登录号
- NP_002403
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途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
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