Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human Insulin-like growth factor I / IGF1 (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % , as determined by Coomassie stained SDS-PAGE
内毒素水平
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 3-15 ng/ml, corresponding to a specific activity of 0.6-3.3 x 105 units/mg, determined by the dose dependent stimulation of MCF-7 cell proliferation.
IGF1
宿主: 人
宿主: HEK-293 Cells
> 95 %
AcA, CC
Active
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
1 x PBS
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.