VCAM1 Protein (Transcript Variant 1)
Quick Overview for VCAM1 Protein (Transcript Variant 1) (ABIN2713362)
抗原
See all VCAM1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human CD106 / VCAM1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0ug/mL.
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Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-697) protein (His tag)
VCAM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (Fc Tag)VCAM1 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-309) protein (His tag)VCAM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag,Fc Tag)VCAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-123) protein (GST tag)VCAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) protein (GST tag)VCAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag)VCAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-698) (Active) protein (His tag)VCAM1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-698) (Active) protein (His tag)VCAM1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, AcA Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-698) protein (His tag)VCAM1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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别名
- Cd106,vcam1
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背景
- This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
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分子量
- 74 kDa
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NCBI登录号
- NP_001069
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途径
- Carbohydrate Homeostasis
抗原
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