P-Selectin 蛋白
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北京 101111
Quick Overview for P-Selectin 蛋白 (ABIN2691046)
抗原
See all P-Selectin (SELP) 蛋白宿主
资源
应用范围
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产品特性
- The Mouse P-selectin - IgG Fusion Protein is composed of the signal sequence, the lectin domain, the EGF-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2 and CH3) of human IgG1. This chimeric protein binds to P-selectin ligand expressed on mouse neutrophils and myeloid cell lines. Furthermore, P-selectin-IgG fusion protein selectively stains Th1 cells, but not Th2 cells.reported in the literature. Detection of P-Selectin ligands on mouse granulocytes (left panel) and mononuclear cells (right panel). BALB/c whole blood was stained with Mouse P-Selectin - IgG Fusion Protein followed by R-PE-conjugated goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, open histogram) or with the second-step alone (filled histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555815/555816), and granulocytes and mononuclear cells were gated according to their light-scatter profiles. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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纯化方法
- Purified
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-771) protein (His-Avi Tag,Biotin)
SELP 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)SELP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-771) protein (Fc Tag)SELP 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 1-829) protein (GST tag)SELP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (Active) protein (His tag)SELP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (His tag,AVI tag)SELP 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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BD Pharmingen™ Purified Mouse P-Selectin - IgG Fusion Protein - Purified - Clone NA - Reactivity Ms
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Avoid multiple freeze-thaws of product. The fusion protein solution should be stored at -20°C until the vial is opened. After opening, undiluted aliquots should be stored at -20°C or below. Thawed aliquots may be stored up to two months at 4°C.
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: "Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis." in: International immunology, Vol. 14, Issue 2, pp. 167-75, (2002) (PubMed).
: "P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues." in: Nature, Vol. 385, Issue 6611, pp. 81-3, (1997) (PubMed).
: "Monospecific and common glycoprotein ligands for E- and P-selectin on myeloid cells." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 471-81, (1994) (PubMed).
: "Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes." in: The Journal of cell biology, Vol. 121, Issue 3, pp. 655-64, (1993) (PubMed).
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: "Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis." in: International immunology, Vol. 14, Issue 2, pp. 167-75, (2002) (PubMed).
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- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
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别名
- P-Selectin
抗原
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