EGFR Protein (GST-His-thrombin cleavage)
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Quick Overview for EGFR Protein (GST-His-thrombin cleavage) (ABIN2669457)
抗原
See all EGFR 蛋白蛋白类型
生物活性
宿主
资源
应用范围
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标记
- This EGFR protein is labelled with GST-His-thrombin cleavage.
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原理
- Recombinant EGFR protein
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产品特性
- Recombinant human EGFR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids H672-A1210, accession number NM_005228 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification. Recombinant EGFR is suitable for kinase assays and Western blot.
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纯化方法
- Purified by GSH-agarose affinity purification.
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Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His-Avi Tag)
EGFR 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor Receptor (EGFR) (AA 25-645) (Active) protein (Biotin,His-Avi Tag)EGFR 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Epidermal Growth Factor Receptor (EGFR) (AA 25-645) (Active) protein (Fc Tag)EGFR 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor Receptor (EGFR) (AA 25-378) (Active) protein (His tag,AVI tag,Biotin)EGFR 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Epidermal Growth Factor Receptor (EGFR) (AA 25-645) (Active) protein (His tag,AVI tag,Biotin)EGFR 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Epidermal Growth Factor Receptor (EGFR) (AA 1-405) protein (GST tag)EGFR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Epidermal Growth Factor Receptor (EGFR) (AA 25-645) protein (His tag)EGFR 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
Epidermal Growth Factor Receptor (EGFR) (AA 25-645) (Active) protein (His tag)EGFR 宿主: 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Epidermal Growth Factor Receptor (EGFR) (AA 669-1210) protein (GST tag)EGFR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Epidermal Growth Factor Receptor (EGFR) (Active) protein (His tag)EGFR 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
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应用备注
- Recombinant EGFR is suitable for kinase assays and Western blot. The molecular weight of the protein is ~91.002 kDa. The activity of the protein is ~ 93 pmol/ug min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 uM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 μg/50 µL PEG20.000, Substrate: PolyEY 10 μg/50 µL, Recombinant EGFR: 40 ng/50 µL. Kinase activity may vary depending on the substrate and reaction conditions used.
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限制
- 仅限研究用
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浓度
- 0.238 μg/μL
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缓冲液
- 10 μg of Recombinant EGFR protein in 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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别名
- EGFR
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背景
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Protein Background: EGFR (Epidermal Growth Factor Receptor) are receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin Isoform 2 may act as an antagonist of EGF action.
Short Description: Recombinant human EGFR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids H672-A1210, accession number NM_005228 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Aliases: ERBB, HER1, mENA, ERBB1, PIG61, NISBD2
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
抗原
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