Interleukin 17a Protein (AA 26-158)
Quick Overview for Interleukin 17a Protein (AA 26-158) (ABIN2667468)
抗原
See all Interleukin 17a (IL17A) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 26-158
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过滤
- 0.22 μm filtered
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内毒素水平
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Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
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Interleukin 17A (IL17A) (AA 24-155) protein (His tag)
IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17A (IL17A) (AA 24-155) protein (His-Avi Tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) protein (His-Avi Tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 92 % as determined by HPLC
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag,Biotin)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag,AVI tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 24-155) protein (His tag)IL17A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 0.25 - 1 ng/ml, corresponding to a specific activity 1.0 - 4 x 106 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- IL-17A
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背景
- IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8. IL-17A shares 58 % homology with an open reading frame of the T-lymphotropic Herpes virus Samirii virus (viral IL-17). IL-17A belongs to a family of cytokines, which has five members, designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells, Th17 cells. This lineage selectively produces proinflammatory cytokines including IL-17, IL-17F, IL-21, and IL-22. In the mouse, the differentiation of this new lineage is initiated by TGFbeta-1 and IL-6 or IL-21, which activates Stat3 and induces the expression of the transcription factor retinoic acid-related orphan receptors (RORγt and RORα). IL-23, which also activates Stat3, serves to maintain Th17 cells lineage.
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分子量
- The 133 amino acid recombinant protein has a predicted molecular mass of 14,978 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,
抗原
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