Interleukin 17a Protein (AA 24-155) (Biotin,His-Avi Tag)
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Quick Overview for Interleukin 17a Protein (AA 24-155) (Biotin,His-Avi Tag) (ABIN7274994)
抗原
See all Interleukin 17a (IL17A) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-155
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标记
- This Interleukin 17a protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human IL-17A/CTLA-8 Protein
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序列
- Gly24-Ala155
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产品特性
- Recombinant Biotinylated Human IL-17A/CTLA-8 Protein is expressed from HEK293 with Avi tag and His tag at the C-Terminus.It contains Gly24-Ala155.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test).,Immobilized Biotinylated Human IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.,Biotinylated Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-17A is greater than 95% as determined by SEC-HPLC.
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Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His-Avi Tag)
IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 17A (IL17A) (AA 26-158) (Active) protein (Biotin,His-Avi Tag)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag,AVI tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 26-158) protein (His tag)IL17A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Interleukin 17A (IL17A) (AA 24-155) protein (His tag)IL17A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Interleukin 17A (IL17A) (AA 24-133) protein (GST tag)IL17A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- IL-17A
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背景
- Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
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分子量
- 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Bis-Tris PAGE result.
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UniProt
- Q16552
抗原
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