Galectin 3 Protein (LGALS3) (AA 2-250)
Quick Overview for Galectin 3 Protein (LGALS3) (AA 2-250) (ABIN2667415)
抗原
See all Galectin 3 (LGALS3) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 2-250
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.1 EU per μg of protein as determine by the LAL method.
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Galectin 3 (LGALS3) (AA 2-250) protein (His tag)
LGALS3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Galectin 3 (LGALS3) (AA 2-264) protein (His tag)LGALS3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Galectin 3 (LGALS3) (AA 2-262) protein (His tag)LGALS3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galectin 3 (LGALS3) (AA 4-262) protein (His tag)LGALS3 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galectin 3 (LGALS3) (AA 1-264) protein (His tag)LGALS3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Galectin 3 (LGALS3) (AA 2-250) protein (His tag)LGALS3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galectin 3 (LGALS3) (AA 1-250) protein (GST tag)LGALS3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Galectin 3 (LGALS3) (AA 1-264) (Active) protein (His tag)LGALS3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active
Galectin 3 (LGALS3) proteinLGALS3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Recombinant human galectin-3 is able to agglutinate human red blood cells with an ED50 of 1.0 - 4.0 μg/ml, corresponding to a specific activity of 2.5 - 10.0 x 105 units/mg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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浓度
- 200 μg/mL
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.3 M NaCl, 2 mM DTT, pH 7.2.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- Galectin 3 (LGALS3)
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别名
- Galectin-3
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物质类
- Chemical
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背景
- Galectin-3 is a member of the lectin family, which includes 15 mammalian lectin members. Like all galectins, galectin-3 contains a carbohydrate recognition binding domain of about 130 amino acids that enables the specific binding of β-galactosides. This protein has been shown to be involved in cell adhesion, cell activation, chemoattraction, cell growth and differentiation, cell cycle, and apoptosis. Galectin-3 induces pro-inflammatory cytokines and inhibits Th2 type cytokine production. It chemoattracts monocytes and macrophages, and activates and degranulates basophils and mast cells. It was reported that galectin-3 knock-out mice demonstrated reduced phagocytic properties in macrophages and Kupffer cells, indicating that this protein is involved in macrophage phagocytosis. Galectin-3 has been shown to be involved in cancer, inflammation, fibrosis, heart disease, and stroke. It has also been shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, tissue repair, inflammation, fibrogenesis, and ventricular remodeling. In addition, galectin-3 was found to contribute to tumor cell evasion of the immune system. Galectin-3 is increasingly being used as a diagnostic marker for different cancers. It can be screened for and used as a prognostic factor to predict the progression of the cancer. Overexpression of galectin-3 is associated with neoplastic transformation, enhancement of tumor cell adhesion to the extracellular matirx, and the subsequent metastatic spreading of cancer cells.
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分子量
- The 270 amino acid recombinant protein has a predicted molecular mass of approximately 28.3 kDa. The protein migrates above 40 kDa by SDS-PAGE in DTT-reducing and non-reducing conditions. The predicted N-terminal amino acid is His.
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途径
- RTK signaling
抗原
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