CD40 Ligand Protein (CD40LG) (AA 114-261)
Quick Overview for CD40 Ligand Protein (CD40LG) (AA 114-261) (ABIN2667388)
抗原
See all CD40 Ligand (CD40LG) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 114-261
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内毒素水平
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Less than 0.1 ng per μg of protein.
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CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (Fc Tag)
CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag,Biotin)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 1-260) protein (His tag)custom-made CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 1-261) protein (His tag)custom-made CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) protein (Fc Tag)CD40LG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG 宿主: 人, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG 宿主: 人 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 5-10 ng/ml, corresponding to a specific activity of 1-2 x 105 units/mg, as determined by the dose-dependent stimulation of IL-8 production by human PBMCs. Results may vary with PBMC donors.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized, carrier-free.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- CD40 Ligand (CD40LG)
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别名
- CD40L
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背景
- CD40L is a type II membrane protein that shares 78 % amino acid identity with its mouse counterpart. Human CD40L is a polypeptide of 261 amino acids, consisting of 22, 24, and 215 amino acids for the cytoplasmic, transmembrane, and extracellular domains respectively. Soluble forms of CD40L (sCD40L) have been identified, and a 18 kD fragment has the ability to form homotrimers and bind to CD40 to induce biological signals. The 18 kD protein has full activity in B cell proliferation and differentiation assays. Activated platelets express CD40L on their surface and constitute the major source of sCD40L (>95 % in blood). Platelet-derived CD40L regulates neutrophil recruitment and lung damage in sepsis, and it has been suggested that MMP-9 regulates CD40L shedding in abdominal sepsis. Platelet-associated CD40L has been linked to progression of atherosclerotic disease, and elevated plasma levels of sCD40L in patients seems to be a reliable predictor of cardiovascular events, such as atherosclerotic plaque rupture and acute coronary syndromes. Also, platelet-associated CD40L expression is increased in idiopathic thrombocytopenic purpura patients, and it has been speculated that platelet-associated CD40L drives the activation of autoreactive B lymphocytes in this disease. In addition, high plasma levels of sCD40L have been connected to HIV-associated neurocognitive disorders (HAND).
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分子量
- The 149 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.3 kDa. The predicted N-terminal amino acid is Met.
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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