NGFB Protein (AA 122-239)
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- 抗原 See all NGFB 蛋白
- NGFB (Nerve Growth Factor beta (NGFB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 122-239
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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Nerve Growth Factor beta (NGFB) (Active) protein
NGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg. Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized, carrier-free.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- NGFB (Nerve Growth Factor beta (NGFB))
- 别名
- beta-NGF (NGFB 产品)
- 别名
- NGFB Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, hsan5 Protein, beta-ngf Protein, Ngfb Protein, Beta-NGF Protein, HSAN5 Protein, beta-NGF Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, beta-Nerve growth factor Protein, nerve growth factor (beta polypeptide) Protein, NGF Protein, ngfb Protein, FPV072 Protein, FPV076 Protein, ngf Protein, Ngf Protein
- 背景
- β-NGF is a pluripotent mediator and it exists in many tissues. Secreted β-NGF can bind to tryosine kinase A receptor with higher affinity and to p75 (NTR) with low affinity. β-NGF is well known as an endogenous neurotrophic factor involved in the embryonal development, survival, and regeneration of mammalian sympathetic and sensory neurons. In adults, β-NGF is also involved in the survival and regeneration of neurons in the central nervous system. Involvement of β-NGF in the immune system has also been reported. In vitro cultured B lymphocyte can constitutively secret β-NGF, which can act as an autocrine growth and differentiation factor for B cells. β-NGF can also downregulate the production of IFN-γ by T cells. Recombinant β-NGF has been used in many clinical trials to treat different disease including Alzheimer's disease, diabetic polyneuropathy, and HIV-associated peripheral neuropathy.
- 分子量
- The 118 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kDa. This protein is a disulfide-linked homodimer and the predicted molecular mass is 27 kDa. The predicted N-terminal amino acid is Ser.
- 途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
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