IGF1 Protein (AA 49-118)
Quick Overview for IGF1 Protein (AA 49-118) (ABIN2666829)
抗原
See all IGF1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 49-118
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.1 EU per μg protein as determined by the LAL method.
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Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein
IGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His tag)IGF1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His-IF2DI Tag)IGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) proteinIGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Insulin-Like Growth Factor 1 (IGF1) (AA 49-138) protein (GST tag)IGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 宿主: 小鼠 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) (AA 1-153) protein (GST tag)IGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 5 - 20 ng/ml, corresponding to a specific activity of 0.05 - 0.20 x 106 units/mg, as determined by the dose dependent stimulation of MCF-7 cell proliferation.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in 300 mM ammonium acetate pH 5.5.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF-I
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背景
- IGF-I (insulin-like growth factor 1), initially described in 1957, is a hormone that is important in childhood growth and also has anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50 % similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I, pro-IGF-I is processed to the mature, active protein, and this procedure requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half life and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs, in serum approximately 75 % of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated to growth hormone insensitivity syndrome (GHIS) also called Laron syndrome, liver cirrhosis, age-related cardiovascular and neurological diseases, and intrauterine growth restriction.
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分子量
- The 70 amino acid recombinant protein has a predicted molecular mass of approximately 7.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 kDa by SDS-PAGE. The N-terminal amino acid is Gly.
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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