HBEGF Protein (AA 63-148)
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Quick Overview for HBEGF Protein (AA 63-148) (ABIN2666821)
抗原
See all HBEGF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 63-148
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 63-148) protein (His-IF2DI Tag)
HBEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 20-160) protein (Fc Tag)HBEGF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 24-160) protein (Fc Tag)HBEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 24-160) protein (His tag)HBEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 63-148) (Active) protein (His tag)HBEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 0.2 - 1.2 ng/ml, corresponding to a specific activity of 0.83 - 5.0 x 106 units/mg, as determined by induction of BALB/3T3 clone A31 cell proliferation.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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别名
- HB-EGF
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背景
- Human HB-EGF was initially identified as a protein of 22 kD secreted by macrophage-like U937 cells. It belongs to the EGF family of proteins that includes EGF, TGF-α, heparin-binding EGF like-growth factor (HB-EGF), epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. It has been suggested that various metalloproteinases participate in the shedding of HB-EGF, such as MMP-3, MMP-7, ADAM9, ADAM10, ADAM12, and ADAM17. The ectodomain shedding is stimulated by phorbol esters, calcium ionophore, lysophosphatidic acid, and IL-1β. In addition, nardilysin, a metalloendopeptidase of the M16 family, binds HB-EGF and enhances its shedding through activation of TACE (ADAM17). The membrane-anchored HB-EGF acts in cell-cell and cell-extracellular matrix interactions, in addition, the membrane HB-EGF is the receptor for diphtheria toxin that can be internalized and induce apoptotic death. HB-EGF plays a crucial role in cardiac valvulogenesis, newborn knockout mice have malformed semilunar and atrioventricular heart valves and poorly differentiated lungs. HB-EGF is overexpressed in several types of cancer such as pancreatic carcinoma, ovarian cancer, and gastric carcinoma. Also, HB-EGF is involved in cancer metastasis and invasion of ovarian cancer, head and neck cancer, and thyroid carcinoma cells.
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分子量
- The 86 amino acid recombinant protein has a predicted molecular mass of approximately 9.7 kDa. The DTT-reduced and non-reduced protein migrate at approximately 13 kDa and 16 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Asp.
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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