IL-5 Protein (AA 21-133)
Quick Overview for IL-5 Protein (AA 21-133) (ABIN2666666)
抗原
See all IL-5 (IL5) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 21-133
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag)
IL5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag,Biotin)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag)IL5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 5 (IL5) (AA 20-134) protein (His tag)IL5 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag,Biotin)IL5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His tag)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 (IL5) (AA 20-134) protein (Fc Tag)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 (IL5) protein (mFc Tag)IL5 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 5 (IL5) protein (His tag)IL5 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 5 (IL5) (AA 20-132) protein (His tag)IL5 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to stimulate the proliferation of BCL1 cells in a dose dependent manner. ED50 = 0.03 - 0.15 ng/ml, corresponding to a specific activity of 0.66 - 3.3 x 107 units/mg.
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限制
- 仅限研究用
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- IL-5
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背景
- IL-5 is a homodimeric glycoprotein that was initially identified by its ability to support the in vitro growth and differentiation of mouse B cells and eosinophils. IL-5 induces eosinophil progenitor cell proliferation, terminal differentiation, and activation. In animal models of allergic diseases or helminth infection, IL-5 induces a massive proliferation of eosinophil progenitors in the bone marrow, promotes eosinophil recruitment with eotaxins, and prolongs eosinophil survival in local tissues. IL-5 regulates genes involved in the B cell terminal differentiation. IL-5 induces CD38-activated splenic B cells to differentiate into immunoglobulin M-secreting cells and go through m to g1 class switch recombination at the DNA level, resulting in immunoglobulin G1 (IgG1) production. IL-5 binds the IL-5R complex, which consists of an IL-5Rα chain specific for IL-5 and a common β-chain that is shared by the receptors for IL-3 and GM-CSF. The alpha subunit is required for ligand-specific binding whereas association with the beta subunit results in increased binding affinity. IL-5 plays important roles in the pathogenesis of asthma, hypereosinophilic syndromes, and eosinophil-dependent inflammatory disease.
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分子量
- The 113 amino acid recombinant protein has a predicted molecular mass of approximately 13.1 kDa. The DTT-reduced protein migrates between 13 to 20 kDa and the non-reduced protein at approximately 40 kDa by SDS-PAGE. The N-terminal amino acid is Met.
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
抗原
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