IL-3 Protein (AA 27-166)
Quick Overview for IL-3 Protein (AA 27-166) (ABIN2666660)
抗原
See all IL-3 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 27-166
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01ng per μg cytokine as determined by the LAL method.
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 0.05 - 0.200 ng/ml, corresponding to a specific activity of 0.5-2.0 x 107 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22μm filtered protein solution is in 10 mM NaHPO4 pH 7.2, 0.15M NaCl
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-3 (Interleukin 3 (IL-3))
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别名
- IL-3
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背景
- IL-3 is the most potent growth factor for basophils followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and rapid and large amount of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis.
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分子量
- The 140 amino acid recombinant protein has a predicted molecular mass of 15673.7 Da. The DTT-reduced protein migrates at approximately 16 kDaa and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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