IL12B Protein (AA 23-335, Homodimer)
Quick Overview for IL12B Protein (AA 23-335, Homodimer) (ABIN2666652)
抗原
See all IL12B 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Homodimer, AA 23-335
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)
IL12B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)IL12B 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 beta (IL12B) protein (His tag)IL12B 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 12 beta (IL12B) (AA 229-328) protein (GST tag)IL12B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)IL12B 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
Interleukin 12 beta (IL12B) (AA 23-328), (full length) protein (GST tag)IL12B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Interleukin 12 beta (IL12B) (AA 23-328) (Active) protein (His tag)IL12B 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 12 beta (IL12B) (AA 23-328) protein (Fc Tag)IL12B 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Interleukin 12 beta (IL12B) (AA 23-327), (full length) protein (His tag)IL12B 宿主: 绵羊 宿主: 酵母菌 Recombinant > 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 1- 4 ng/ml corresponding to a specific activity of 1.0 - 0.25 x 106 units/mg, as determined by the dose dependent inhibition of IL-12-dependent IFNγ production in splenocytes.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL12B (Interleukin 12 beta (IL12B))
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别名
- IL-12 p40
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背景
- IL-12 and IL-23 share the p40 subunit, which heterodimerizes respectively with IL-12 p35 or IL-23 p19 subunits to form IL-12 or IL-23. IL-12 p40 exists as a monomer and as a homodimer (IL-12 p80). IL-12 induction is relevant in asthmatic airway inflammation. IL-12 expression can be induced by mouse parainfluenza type I (Sendai) virus and its source is airway epithelial cells. In that experimental model, IL-12 induction is followed by excessive expression of IL-12 p40 that could be further enhanced in IL-12 p35-deficient mice. Overexpression of IL-12 p80 causes macrophage accumulation and contributes to airway inflammation and consequent morbidity during viral bronchitis. Amplified epithelial IL-12 p40 expression and augmented concentrations of BAL fluid IL-12 p40 (but not IL-12 p70) has been detected in asthmatic subjects. It has been demonstrated that p80, but not IL-12 or p40, induces macrophage chemotaxis that is independent of IL-12 and mediated through the cytoplasmic tail of IL-12b1. Additional studies with transgenic mice suggest that overexpression of IL-12 p80 prior to a viral infection increases the number of resident airway macrophages, and this primes the host for a protective response against a lethal respiratory viral infection. In addition, it has been suggested that p80 functions as a competitive antagonist of IL-12 p70. Mouse Con A-activated splenocytes display identical binding affinities for p80 and IL-12, and in these cells p80 competitively inhibited IL-12 binding and IL-12-dependent proliferation. Furthermore, p80 is able to inhibit IL-12-dependent IFNγ production in freshly isolated splenocytes.
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分子量
- The 313 amino acid recombinant protein has a predicted molecular mass of 35.8 kDa. The DTT-reduced protein migrates at approximately 40 kDa and the non-reduced protein migrates at approximately 75 kDa by SDS-PAGE. The N-terminal amino acid is Met.
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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