IL-3 Protein (AA 27-169)
Quick Overview for IL-3 Protein (AA 27-169) (ABIN2666554)
抗原
See all IL-3 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 27-169
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 27-166) protein (His-IF2DI Tag)IL-3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 1 - 5 ng/ml, corresponding to a specific activity of 0.2 â€" 1.0 x 106 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-3 (Interleukin 3 (IL-3))
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别名
- IL-3
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背景
- Interleukin 3, also known as IL-3, was initially described in the supernatant of cultures of viral-infected murine spleen cells. Mature rat IL-3 surprisingly shows low homology (54 % ) with its murine counterpart. IL-3 is the most potent growth factor for basophils followed by the granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and release of the cells. IL-3 is highly expressed by mast cells. Rapid autocrine production of large amounts of the cytokine is responsible for mast cell IgE-induced survival in the absence of antigen. In addition, IL-3 and its receptors are expressed in neuronal progenitors and neurons. IL-3 has been associated to human brain volume variation by regulating proliferation and survival of neural progenitors. It has also been implicated in the pathogenesis of several chronic inflammatory diseases, which include asthma, atherosclerosis, and neurodegenerative disorders such as multiple sclerosis.
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分子量
- The 144 amino acid recombinant protein has a predicted molecular mass of approximately 16.3 kDa. The protein migrates at approximately 18 kDa in DTT-reducing conditions and 16 kDa in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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