Cathepsin B Protein (CTSB) (AA 18-339)
Quick Overview for Cathepsin B Protein (CTSB) (AA 18-339) (ABIN2666495)
抗原
See all Cathepsin B (CTSB) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 18-339
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01ng per μg cytokine, as determined by the LAL method.
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Cathepsin B (CTSB) (AA 18-339) protein (His tag)
CTSB 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
Cathepsin B (CTSB) protein (His tag)CTSB 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Cathepsin B (CTSB) (AA 18-339) (Active) protein (His tag)CTSB 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, EAA Active
Cathepsin B (CTSB) (AA 1-339) protein (GST tag)CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) (AA 21-339) protein (GST tag)CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) (Active) protein (His tag)CTSB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Cathepsin B (CTSB) protein (His tag)CTSB 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Cathepsin B (CTSB) (AA 82-333), (partial) protein (GST tag)CTSB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Cathepsin B (CTSB) (AA 18-339) protein (His tag)CTSB 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The activity of CTSB is determined by its ability to cleave the fluorogenic peptide substrate, Z-Leu-Arg-AMC (Z=Benzyloxycarbonyl, AMC=7-amino-4-methylcoumarin) after stimulation. The specific activity is >2,500 pmol/min/μg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22μm filtered protein solution is in 20 mM Tris, 100 mM NaCl, and pH 8.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -70°C for six months.
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- Cathepsin B (CTSB)
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别名
- Cathepsin B
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背景
- Cathepsin B (CTSB) is a lysosomal cysteine protease. While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. The optimal pH for CTSB activity is between four and six. Cystatin C has been identified as an endogenous CTSB inhibitor. High CTSB protein levels and activities have been found in many tumors including breast, cervix, colon, stomach, glioma, lung and thyroid tumors. CTSB can be secreted by tumor cells and is associated with the cell membrane of these cells. Membrane associated CTSB promotes extracellular matrix (ECM) degradation contributing to cancer motility and invasion. Many ECM proteins, including laminin, fibronectin, and collagen IV, are substrates of CTSB. CTSB can also activate pro-uPA/PLAU. Activated uPA promotes ECM digestion through serine protease plasminogen. It has been shown that the inhibition of CTSB can limit bone metastasis in breast cancer, making it an important anti-cancer drug target. CTSB has been proposed as a new drug target for Alzheimer's disease because of its involvement in the production of neurotoxic β -amyloid (Aβ) peptides. The inhibition of CTSB can reduce the brain Aβ peptides and improve memory determined from a mouse model with Alzheimer's disease. CTSB also plays significant roles in immune responses including both T and B cell apoptosis and Th1/Th2 polarization. CTSB is implicated in other pathological conditions including cardiovascular disease, multiple sclerosis, and arthritis. Roles of CTSB in autophagy, adipogenesis, and cholesterol absorption in the intestine have also been studied.
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分子量
- The 332 amino acid recombinant protein has a predicted molecular mass of approximately 37 kDa. The DTT-reduced and non-reduced protein migrate at approximately 45 kDa by SDS-PAGE. The predicted N-terminal amino acid is Arg.
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途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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