FGF6 Protein (AA 41-208, N-Term)
This Recombinant FGF6 protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for FGF6 Protein (AA 41-208, N-Term) (ABIN2666435)
抗原
See all FGF6 蛋白
FGF6
(Fibroblast Growth Factor 6 (FGF6))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
纯度
> 95 % , as determined by Coomassie stained SDS-PAGE.
-
-
产品特性
- N-Term, AA 41-208
-
过滤
- 0.22 μm filtered
-
内毒素水平
-
Less than 0.01 ng per μg cytokine as determined by the LAL method.
-
-
Fibroblast Growth Factor 6 (FGF6) (AA 41-208) protein (His-IF2DI Tag)
FGF6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Biological activity: The ED50 is 0.02 - 0.1 ng/mL, corresponding to a specific activity 1.0 - 5.0 x 107 units/mg, as determined by a dose-dependent proliferation of NIH3T3 cells in the presence of 1.5 μg/mL heparin.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.
-
缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2, 10 % Glycerol and 1 % CHAPS.
-
注意事项
- Avoid repeated freeze/thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- Human FGF-6 is not stable at 37°C. Unopened vials can be stored between 2°C and 8°C for one week, at -20°C for three months, or at -70°C for 6 months.
-
-
- FGF6 (Fibroblast Growth Factor 6 (FGF6))
-
别名
- FGF-6
-
背景
- FGF-6 is a member of the fibroblast growth factor (FGF) family, and it is closely related in structure to FGF-4. FGF-6 exhibits a very restricted expression profile predominantly in the developing muscle in embryos. FGF-6 mRNA can also be detected in testis, heart, and skeletal muscle in adults. FGF-6 is involved in both proliferation and differentiation of the myogenic lineages. In vitro studies have shown that the effects of FGF-6 are concentration dependent. At lower concentration, FGF-6 can increase the expression of many muscle cell differentiation markers. At higher concentrations, FGF-6 can downregulate the expression of myogenic markers and some myogenic transcription factors, and can delay C2 myogenic cell differentiation. Injection of FGF-6 recombinant protein into damaged mouse muscle can induce cyclin D1 mRNA and differentiation markers, including Cdkl1, MHC I and Tn1, which results in the acceleration of muscle regeneration. It has been shown that the expression of FGF-6 can transiently be upregulated by muscle injuries in both fast and slow myofibers. FGF-6 can stimulate migration of myogenic stem cells and also control the maintenance of muscle progenitor cells. During muscle regeneration, it has been shown that FGF-6 can be released from necrotic myofibers and be sequestered by basal laminae. FGF-6 regulates muscle differentiation through a calcineurin-dependent pathway and regulates myofiber size through IGF-II/TGF2R pathway. FGF-6 is also involved in bone metabolism.
-
分子量
- The 169 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kDa. The DTT-reduced and non-reduced protein migrates at approximately 20 kDa by SDS-PAGE. The predicted N-terminal amino acid is Met.
-
途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
-
You are here:
