ED50 <10 ng/mL, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of >1×10^5 units/mg. AA 49-118, expressed with an N-terminal Met.
Lyophilized recombinant mouse Insulin-like Growth Factor I (rmIGF-I) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmIGF-I remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Insulin-like Growth Factor I (IGF-I) is a single chain 7 kDa growth-promoting polypeptide originally identified as somatomedin-c. It belongs to the IGF family of peptides, which also includes IGF-II and insulin. The gene expression of IGF-I is mainly regulated by Growth Hormone, and IGF-I executes its functions via signaling through transmembrane tyrosine receptors (IGF Receptors). Most circulating IFG-I is associated with the IGF Binding Protein 3 (IGFBP-3), and the IGFBPs may inhibit the actions of IGFs by competing against the IGF Receptors. IGF-I is active in post-natal and adult animals, and is crucial for somatic growth, as IGF-I null mice show marked retardation in utero. IGF-I is involved in carcinogenesis, and related to prostate cancer as well.Recombinant mouse Insulin-like Growth Factor I (rmIGF-I) produced in E. coli is a single non-glycosylated polypeptide chain containing 71 amino acids. A fully biologically active molecule, rmIGF-I has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE. Synonyms: Insulin-like Growth Factor-I, Somatamedin C, IGF-IA