HGF Protein (AA 32-494, AA 495-728)
Quick Overview for HGF Protein (AA 32-494, AA 495-728) (ABIN2017955)
抗原
See all HGF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 32-494, AA 495-728
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产品特性
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ED50 < 10 ng/mL, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1x10^5 units/mg.
Alpha chain: AA 32-494, beta chain: AA 495-728 -
内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (His tag)
HGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) protein (Fc Tag)HGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-285) proteinHGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS - PAGE SDS
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 496-728) protein (His-IF2DI Tag)HGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 493-721) protein (His-IF2DI Tag)HGF 宿主: 小鸡 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 619-728) protein (GST tag)HGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) (Active) protein (His tag)HGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 32-728) (Active) protein (Fc Tag)HGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 477-728) protein (GST tag)HGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in ddH2O or PBS at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against PBS.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant humanHepatocyte Growth Factor (HGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human Hepatocyte Growth Factor (HGF) should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
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有效期
- 6 months
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- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
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别名
- Hepatocyte Growth Factor (HGF)
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背景
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Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.Recombinant human Hepatocyte Growth Factor (rhHGF) is produced in CHO cells and consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in the formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues. A fully biologically active molecule, rhHGF has a molecular mass of around 88-90 kDa analyzed by reducing SDS-PAGE.
Synonyms: -
分子量
- 88-90 kDa (single chain), 59-61kDa (alpha chain), 30-34kDa (beta chain), observed by reducing SDS-PAGE.
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途径
- RTK signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原
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