HBEGF 蛋白
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北京 101111
Quick Overview for HBEGF 蛋白 (ABIN2017928)
抗原
See all HBEGF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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序列
- DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
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产品特性
- Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1 EU/μg of rHuHB-EGF as determined by LAL method.
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Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 24-160) protein (Fc Tag)
HBEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 63-148) protein (His-IF2DI Tag)HBEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 20-160) protein (Fc Tag)HBEGF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 24-160) protein (His tag)HBEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 63-148) (Active) protein (His tag)HBEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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缓冲液
- Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
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- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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别名
- HB-EGF
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背景
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Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene.HB-EGF-like growth factor is synthesized as a membrane-anchored mitogenic and chemotactic glycoprotein. An epidermal growth factor produced by monocytes and macrophages, due to an affinity for heparin is termed HB-EGF. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. First identified in the conditioned media of human macrophage-like cells, HB-EGF is an 87 amino acid gylcoprotein which displays highly regulated gene expression.Ectodomain shedding results in the soluble mature form of HB-EGF which influences the mitogenicity and chemotactic factors for smooth muscle cells and fibroblasts. The transmembrane form of HB-EGF is the unique receptor for diptheria toxin and functions in juxtacrine signaling in cells. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HB-EGF can bind two locations on cell surfaces, heparan sulfate proteoglycans and EGF-receptor effecting cell to cell interactions.
Synonyms: Heparin Binding EGF-like growth factor, HBEGF, Diphtheria toxin receptor, DTR -
分子量
- 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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