KIT Ligand Protein (KITLG)
Quick Overview for KIT Ligand Protein (KITLG) (ABIN1589652)
抗原
See all KIT Ligand (KITLG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- SCF
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序列
- MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
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特异性
- Chromosomal location:10 D1, 10 57.0 cM
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产品特性
- Length (aa):165
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内毒素水平
- < 0.1 ng per μg of mSCF
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KIT Ligand (KITLG) (Active) protein
KITLG 宿主: 人 宿主: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) (AA 26-189) (Active) proteinKITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is in the range of 2-10 ng/mL.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Mouse SCF should be reconstituted in 50 mM acetic acid or water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions or stored at -20 °C.
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缓冲液
- 50 mM aceetic acid
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注意事项
- Avoid repeated freeze-thaw cycles. Freeze/thaw cycles will result in significant loss of activity.
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储存条件
- 0 °C
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储存方法
- The lyophilized mouse SCF is best stored desiccated below 0°C. Freeze/thaw cycles will result in significant loss of activity. Avoid repeated freeze-thaw cycles.
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- KIT Ligand (KITLG)
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别名
- SCF
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背景
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Stem cell factor (SCF), also known as ckit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform, mouse SCF shares 93% aa sequence identity with rat SCF and 72 %-75 % with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/ckit to trigger receptor dimerization and signaling.
Synonyms: Kitl, Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, Kitlg, contrasted -
分子量
- 18.42 kDa
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基因ID
- 17311
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NCBI登录号
- NM_013598, NP_038626
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UniProt
- P20826
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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