KIT Ligand Protein (KITLG)
Quick Overview for KIT Ligand Protein (KITLG) (ABIN1589635)
抗原
See all KIT Ligand (KITLG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- SCF
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序列
- MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
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特异性
- Chromosomal location:12q22
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产品特性
- Length (aa):165
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内毒素水平
- < 0.1 ng per μg of human SCF
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KIT Ligand (KITLG) (Active) protein
KITLG 宿主: 人 宿主: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) (AA 26-189) (Active) proteinKITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is in the range of 1 - 5 ng/mL. The WHO standard #91/682 was used as a control.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Human SCF should be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be further diluted in water or other buffer solutions or stored at -20 °C.
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缓冲液
- PBS
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储存条件
- RT,4 °C,-20 °C
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储存方法
- Lyophilized SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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- KIT Ligand (KITLG)
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别名
- SCF
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背景
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Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acids (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternatively spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72 % to 75 % with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
Synonyms: KITLG, SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, kit-ligand, stem cell factor, Mast cell growth factor, c-Kit ligand -
分子量
- 18.5 kDa
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基因ID
- 4254
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NCBI登录号
- NM_000899, NP_000890
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UniProt
- P21583
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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