VEGF121 Protein (Homodimer)
Quick Overview for VEGF121 Protein (Homodimer) (ABIN1589588)
抗原
See all VEGF121 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- Homodimer
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原理
- VEGF121
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序列
- APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKCDKPR R
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特异性
- Chromosomal location:6p12
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产品特性
- Length (aa):121
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Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) protein (His-Avi Tag,Biotin)
VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) protein (His-Avi Tag)VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) (Active) proteinVEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 28-147) (Active) protein (His tag)VEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (Isoform 121) proteinVEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS PAGE SDS
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应用备注
- Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 2 - 10 ng/mL.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized VEGF121 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
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缓冲液
- 50 mM acetic acid
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储存条件
- -20 °C,-80 °C
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储存方法
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF121 should be stored in working aliquots at -20°C.
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有效期
- 6 months
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- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
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别名
- VEGF121
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背景
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Human Vascular Endothelial Growth Factor121 (VEGF121), a 18 kDa protein consisting of 121 amino acid residues is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Synonyms: vascular endothelial growth factor A 121, VEGFA, VPF, VEGF, MVCD1, -
分子量
- 36 kDa
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基因ID
- 7422
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NCBI登录号
- NM_001025366, NP_001020537
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UniProt
- P15692
抗原
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