(3 references)-
- 抗原 See all IL-3 蛋白
- IL-3 (Interleukin 3 (IL-3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity: The activity range is measured in a proliferation assay using TF-1 cells.
Specific Activity: 0.3 - 1.4 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.)
ED50: 70-300 pg/ml
Observed dose response: > 200 fold - 产品特性
- Recombinant human IL-3 is supplied as a frozen aqueous buffered solution containing glycerol, 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and with no preservatives. Recombinant human IL-3 is > 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant human IL-3 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing glycerol and bovine serum albumin, with no preservatives. Recombinant human IL-3 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of human IL-3, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IL-3 蛋白
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (Fc Tag)IL-3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 20-152) (Active) proteinIL-3 宿主: 人 宿主: Tobacco (Nicotiana benthamiana) Recombinant WB, CC Active
Interleukin 3 (IL-3) (AA 20-152) protein (His tag)IL-3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 3 (IL-3) protein (His tag)IL-3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
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- 应用备注
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ELISA Standard: Recombinant human IL-3 is useful as a quantitative standard for measuring human IL-3 protein levels in an IL-3 specific sandwich ELISA with the purified BVD8-3G11 antibody (Cat. No. 554672) as a capture antibody and the biotinylated BVD3-1F9 (Cat. No. 554674) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-3 standard from 2000 to 15 pg/ml should be included in each ELISA plate.
Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant human IL-3 can be used as a blocking control to demonstrate specificity of IL-3 staining by the directly conjugated-BVD3 1F9 antibody (Cat. No. 554676). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook both of which are posted on our web site. - 说明
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BD Pharmingen™ Recombinant Human IL-3 - Reactivity Hu
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA and glycerol.
- 储存液
- Azide free
- 注意事项
- Interleukin-3 (IL-3) is a species specific colony stimulating factor which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15 kD protein containing 133 amino acid residues.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Interleukin 3: from colony-stimulating factor to pluripotent immunoregulatory cytokine." in: International journal of immunopharmacology, Vol. 14, Issue 3, pp. 421-30, (1992) (PubMed).
: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- 抗原
- IL-3 (Interleukin 3 (IL-3))
- 别名
- IL-3 (IL-3 产品)
- 别名
- BPA Protein, Csfmu Protein, HCGF Protein, Il-3 Protein, MCGF Protein, PSF Protein, IL-3 Protein, MULTI-CSF Protein, interleukin 3 Protein, interleukin 3 (colony-stimulating factor, multiple) Protein, Il3 Protein, IL3 Protein
- 背景
- Interleukin-3 (IL-3) is a species specific colony stimulating factor which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15 kD protein containing 133 amino acid residues.
- 途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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