HMGB1 Protein (BoxA)
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北京 101111
Quick Overview for HMGB1 Protein (BoxA) (ABIN1176814)
抗原
See all HMGB1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- BoxA
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原理
- BoxA is one of the highly conserved DNA binding domains of HMGB1 protein (HMG boxes), and inhibits HMGB1 effects in vivo.
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序列
- BoxA from HMGB1 consists of 89 amino acid: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF
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产品特性
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The BoxA we provide has no difference from the sequence of the human or mouse HMGB1, and as a self-antigen elicits very low immunological responses.
BoxA protects against sepsis in a mouse peritonitis model (Yang et al. 2004), and from hepatitis in a mouse model of HBV infection (Sitia et al. 2007).
Dendritic cells promote their own maturation and T cell proliferation, survival and Th1 differentiation by secreting HMGB1, and BoxA inhibits these activities (Dumitriu et al. 2005).
BoxA also inhibits the chemotactic activity of HMGB1 and the mitogenic activity of HMGB1 on stem cells.
BoxA from HMGB1 is produced in E.coli from an expression plasmid coding for the unmodified mammalian sequence, which is totally identical in human and mouse. -
纯化方法
- Purified. The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure.
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内毒素水平
- LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (Biotin,His tag)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
High Mobility Group Box 1 (HMGB1) (C106S), (C23S), (C45S), (Non-oxidizable) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % It contains no nucleic acids. CA, Act Active
High Mobility Group Box 1 (HMGB1) (Fully reduced) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % It contains no nucleic acids. CA, Act Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 宿主: 人 宿主: Baculovirus Recombinant > 90 % pure SDS
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- the lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
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缓冲液
- 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT. The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure. The remaining traces of Triton X-114 can be removed upon request.
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注意事项
- The product is resistant to repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- 2-8°C. The protein once resuspended can be stored frozen (-20°C). The product is resistant to repeated freezing and thawing.
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: "Toll-like receptor 4 and high-mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizures." in: Nature medicine, Vol. 16, Issue 4, pp. 413-9, (2010) (PubMed).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- High-mobility group protein B1 (HMGB1)
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分子量
- 10.4 kDa
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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