C5A Protein (AA 679-755)
Quick Overview for C5A Protein (AA 679-755) (ABIN1096418)
抗原
See all C5A (C5a) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 679-755
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原理
- Recombinant Mouse Complement Component C5
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序列
- MNLHLLRQKI EEQAAKYKHS VPKKCCYDGA RVNFYETCEE RVARVTIGPL CIRAFNECCT IANKIRKESP HKPVQLGR
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产品特性
- Recombinant Mouse Complement C5a produced in E. coli is a single non-glycosylated polypeptide chain containing 78 amino acids (N679-R755) with a molecular mass of 9 kDa.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Complement Component 5a (C5a) (AA 678-751) protein (His tag)
C5a 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Complement Component 5a (C5a) (AA 1-77) protein (His-IF2DI Tag)C5a 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Complement Component 5a (C5a) (AA 679-751) proteinC5a 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE.
Complement Component 5a (C5a) (AA 679-751) (Active) protein (Fc Tag)C5a 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Complement Component 5a (C5a) (AA 679-755) (Active) protein (His tag)C5a 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Complement Component 5a (C5a) (AA 678-751) (Active) proteinC5a 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 350 mM NaCl, pH 7.5.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- C5A (C5a) (Complement Component 5a (C5a))
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别名
- c5a
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背景
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Mouse Complement C5 (C5a) is a glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 77 amino acid peptide that is created by the C5a convertase proteolytic cleavage of C5 alphachain in the classical and alternative complement pathway (C4b2a3b, C3bBb3b). Mouse C5a has fouralphahelices, plus three intra-chain disulfide bonds that form a triple loop structure. C5a functions via G-protein coupled receptor (GPCR) (C5aR/CD88). C5a is a potent chemoattractant and anaphylatoxin that acts on all classes of leukocytes and on many other cell types including endothelial, smooth muscle, kidney, liver, and neural cells. It mediates IL-8 release from bronchial epithelial cells. It also triggers an oxidative burst in macrophages and neutrophils, causing release of histamine in basophils and mast cells. C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion. Mouse C5a shares 60% and 82% sequence identity to human and rat C5a, respectively.
Alternative Names: Complement C5, Hemolytic Complement, C5, Hc -
分子量
- 9 kDa
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UniProt
- P06684
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途径
- Complement System, Carbohydrate Homeostasis
抗原
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