UNG 蛋白
Quick Overview for UNG 蛋白 (ABIN1068292)
抗原
See all UNG 蛋白生物活性
宿主
资源
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产品特性
- 10x UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.
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过滤
- Sterile filtered
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Unit Definition
- 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.
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Uracil-DNA Glycosylase (UNG) (AA 1-313) protein (His tag)
custom-made UNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Uracil-DNA Glycosylase (UNG) (AA 1-304) protein (GST tag)UNG 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Uracil-DNA Glycosylase (UNG) (AA 1-229) protein (His tag)UNG 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Uracil-DNA Glycosylase (UNG) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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说明
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Specific Activity: The Specific Activity was found to be 5U/μl.
Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Inhibition and Inactivation: Inactivated by heating at 95°C for 10min. Enzye activity is partially restored at temperatures lower than 55°C. -
限制
- 仅限研究用
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缓冲液
- UNG solution(5U/ul)in 10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
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储存方法
- Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
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- UNG (Uracil-DNA Glycosylase (UNG))
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别名
- Uracil DNA Glycosylase
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物质类
- Viral Protein
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背景
- E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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