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Protein Kinase A, alpha (PRKACA) (N-Term) Peptide

PRKACA 适用: 人 宿主: 合成 BP, Imm
产品编号 ABIN5539428
发货至: 中国
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Quick Overview for Protein Kinase A, alpha (PRKACA) (N-Term) Peptide (ABIN5539428)

抗原

PRKACA (Protein Kinase A, alpha (PRKACA))

宿主

资源

  • 8
合成

应用范围

Blocking Peptide (BP), Immunogen (Imm)
  • 蛋白结构域

    N-Term

    序列

    ESPAQNTAHL DQ

    预测反应

    Human
  • 应用备注

    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 48 kDa band observed in lysates of cell line HeLa and MCF7 (calculated MW of 49.1 kDa according to NP_002721.1). Recommended concentration: 1-3 μg/mL.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.

    缓冲液

    100µg of Lyophilised peptide, formulation De-ionized (DI) water.

    储存条件

    -20 °C

    储存方法

    Shipped at ambient temperature, store at -20°C.
  • 抗原

    PRKACA (Protein Kinase A, alpha (PRKACA))

    基因ID

    5566

    NCBI登录号

    NP_002721, NP_997401, NP_001291278
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