Protein Kinase A, alpha (PRKACA) (N-Term) Peptide
Our Local Distributor
北京 101111
Quick Overview for Protein Kinase A, alpha (PRKACA) (N-Term) Peptide (ABIN5539428)
抗原
宿主
资源
应用范围
-
-
蛋白结构域
- N-Term
-
序列
- ESPAQNTAHL DQ
-
预测反应
- Human
-
-
-
-
应用备注
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 48 kDa band observed in lysates of cell line HeLa and MCF7 (calculated MW of 49.1 kDa according to NP_002721.1). Recommended concentration: 1-3 μg/mL.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
-
缓冲液
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
-
储存条件
- -20 °C
-
储存方法
- Shipped at ambient temperature, store at -20°C.
-
-
- PRKACA (Protein Kinase A, alpha (PRKACA))
-
基因ID
- 5566
-
NCBI登录号
- NP_002721, NP_997401, NP_001291278
抗原
-