CCL2 ELISA 试剂盒
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北京 101111
Quick Overview for CCL2 ELISA 试剂盒 (ABIN955781)
抗原
See all CCL2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 74 pg/mL
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Analytical Method
- Quantitative
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特异性
- The assay detects both natural and recombinant human MCP-1. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MCP-1 positive serum. There was no cross reactivity detected, namely not with MCP-3.
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灵敏度
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74 pg/mL.
The limit of detection of human MCP-1 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined - be 2.31 pg/mL (mean of 6 independent assays). -
产品特性
- Coated Microwell - Monoclonal Coating Antibody An anti-human MCP-1 coating antibody is adsorbed onto microwells. Human MCP-1 present in the sample or calibrator binds to antibodies adsorbed to the microwells and the HRP-conjugated anti-human MCP-1 antibody is added and binds to human MCP-1 captured by the first antibody. Following incubation unbound HRP-conjugated anti-human MCP-1 is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of human MCP-1 present in the sample or calibrator. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A calibration curve is prepared from 7 human MCP-1 calibrator dilutions and human MCP-1 concentration determined.
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组件
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1 aluminium pouch with a Microwell Plate coated with monoclonal antibody to human MCP-1
1 vial (100 µL) HRP-Conjugate anti-human MCP-1 monoclonal antibody
2 vials human MCP-1 Calibrator lyophilized, 2 ng/mL upon reconstitution
1 vial Control high, lyophilized
1 vial Control low, lyophilized
1 bottle (50 mL) Wash Buffer Concentrate 20X (PBS with 1% Tween 20)
1 vial (5 mL) Assay Buffer Concentrate 20X (PBS with 1% Tween 20 and protein stabilizer)
1 vial (15 mL) Substrate Solution (tetramethyl-benzidine)
1 vial (15 mL) Stop Solution (1M Phosphoric acid)
1 vial (0.4 mL) Blue-Dye
1 vial (0.4 mL) Green-Dye
2 adhesive Plate Covers. -
试剂未包括
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5 mL and 10 mL graduated pipettes
5 µL to 100 µL adjustable single channel micropipettes with disposable tips
50 µL to 300 µL adjustable multichannel micropipette with disposable tips
Multichannel micropipette reservoir
Beakers, flasks, cylinder
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Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
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Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 兔 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 小鼠 AA 24-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
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板类型
- Pre-coated
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试剂准备
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Buffer Concentrates should be brought to room temperature and should be diluted before starting the test procedure. If crystals have formed in the Buffer Concentrates, warm them gently until they have completely dissolved.
Wash Buffer (1X): Pour entire contents (50 mL) of the Wash Buffer Concentrate (20X) into a clean 1,000 mL graduated cylinder. Bring to final volume of 1,000 mL with glass-distilled or de-ionized water. Mix gently to avoid foaming. Transfer to a clean wash bottle and store at 2°to 25°C. Please note that the Wash Buffer (1X) is stable for 30 days.
Assay Buffer (1X) Pour the entire contents (5 mL) of Assay Buffer Concentrate (20X) into a clean 100 mL graduated cylinder. Bring to final volume of 100 mL with distilled water. Mix gently to avoid foaming. Store at 4°C. Please note that the Assay Buffer (1X) is stable for 30 days.
HRP-Conjugate (Please note that the HRP-Conjugate should be used within 30 minutes after dilution.): Make a 1:100 dilution of the concentrated HRP-Conjugate solution with Assay Buffer (1X) in a clean plastic test tube as needed.
Human MCP-1 Calibrator: Reconstitute human MCP-1 calibrator by addition of distilled water. Reconstitution volume is stated on the label of the calibrator vial. Swirl or mix gently to insure complete and homogeneous solubilization (concentration of reconstituted calibrator = 2 ng/mL). Allow the calibrator to reconstitute for 10-30 minutes. Mix well prior to making dilutions. After usage remaining calibrator cannot be stored and has to be discarded. Calibrator dilutions can be prepared directly on the microwell plate or alternatively in tubes.
External Calibrator Dilution: Label 7 tubes, one for calibrator point. C1, C2, C3, C4, C5, C6, C7 Then prepare 1:2 serial dilutions for the calibration curve as follows: Pipette 225 µL of Assay Buffer (1X) into each tube. Pipette 225 µL of reconstituted calibrator (concentration of calibrator = 2 ng/mL) into the first tube, labeled C1, and mix (concentration of calibrator 1 = 1 ng/mL). Pipette 225 µL of this dilution into the second tube, labeled C2, and mix thoroughly before the next transfer. Repeat serial dilutions 5 more times thus creating the points of the calibration curve. Assay Buffer (1X) serves as blank.
Controls: Reconstitute by adding 150 µL distilled water to lyophilized controls (10-30 minutes). Further treat the controls like your samples in the assay. For control range please refer to vial label. Store reconstituted controls aliquoted at -20°C. Avoid repeated freeze and thaw cycles.
Addition of Color-giving Reagents: Blue-Dye, Green-Dye: In order to help our customers to avoid any mistakes in pipetting the Human MCP-1 ELISA, now offers a tool that helps to monitor the addition of even very small volumes of a solution to the reaction well by giving distinctive colors to each step of the ELISA procedure. This procedure is optional, does not in any way interfere with the test results, and is designed to help the customer with the performance of the test, but can also be omitted, just following the instruction booklet. -
样品制备
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Cell culture supernatant, serum, plasma (EDTA, heparin) and amniotic fluid were tested with this assay. Other biological samples might be suitable for use in the assay. Remove serum or plasma from the clot or red cells as soon as possible after clotting and separation. Pay attention to a possible Hook Effect due to high sample concentrations. Samples containing a visible precipitate must be clarified prior to use in the assay. Do not use grossly hemolyzed or lipemic specimens. Samples should be aliquoted and must be stored frozen at -20°C to avoid loss of bioactive human MCP-1. If samples are to be run within 24 hours, they may be stored at 4°C. Avoid repeated freeze-thaw cycles. Prior to assay, the frozen sample should be brought to room temperature slowly and mixed gently.
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限制
- 仅限研究用
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注意事项
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Since exact conditions may vary from assay to assay, a calibration curve must be established for every run.
Bacterial or fungal contamination of either screen samples or reagents or cross-contamination between reagents may cause erroneous results. -
储存条件
- -20 °C
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储存方法
- Aliquots of serum samples (spiked or unspiked) were stored at -20°C and thawed 5 times, and the human MCP-1 levels determined. There was no significant loss of human MCP-1 immunoreactivity detected by repeated freezing and thawing. Store kit reagents at 4°C except controls. Store lyophilized controls at -20°C. Immediately after use remaining reagents should be returned to cold storage (4°C), or to -20°C, respectively. Expiry of the kit and reagents is stated on labels. Expiry of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.
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- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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别名
- MCP-1
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背景
- Chemokines (chemoattractant cytokines) represent a superfamily of small secreted proteins that function as intercellular messengers to control migration and activation of leukocytes involved in inflammatory reactions and immunity. Furthermore, chemokines are important mediators of many pathologies such as allergic responses, chronic inflammatory and autoimmune diseases, tumor growth and hematopoietic development. The CC-chemokine monocyte chemoattractant protein 1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells. The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF- and IL-1, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. Elevated levels of MCP-1 have also been found in connection with osseus inflammation and Alzheimer's disease (AD) as well as Myocardial Ischemia and viral infections. In acute and chronic-active multiple sclerosis (MS) lesions immunoreactivity for MCP-1 was increased whereas MCP-1 was found to be significantly reduced in cerebrospinal fluids (CSF) and chronic lesions of patients with MS. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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