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CXCL2 ELISA 试剂盒

Name: CXCL2 ELISA 试剂盒
SKU: ABIN921067
Availability: OutOfStock

This 小鼠 CXCL2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CXCL2. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN921067

发货至: 中国

Datasheet as PDF

Quick Overview for CXCL2 ELISA 试剂盒 (ABIN921067)

抗原

See all CXCL2 ELISA试剂盒

CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

抗原表位

AA 28-100

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product CXCL2 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MIP-2

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: A28-N100

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<5pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: A28-N100
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Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
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Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
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Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 大鼠 Colorimetric Sandwich ELISA 0.03-2.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MIP-2 has been precoated onto 96-well plates. Standards(E.coli, A28-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MIP-2 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MIP-2 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 113, Standard deviation: 4.41, CV(%): 3.9
- Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 17.75, CV(%): 5
- Sample 3: n=16, Mean(pg/ml): 627, Standard deviation: 40.13, CV(%): 6.4,
- Sample 1: n=24, Mean(pg/ml): 134, Standard deviation: 8.31, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 387, Standard deviation: 21.3, CV(%): 5.5
- Sample 3: n=24, Mean(pg/ml): 653, Standard deviation: 44.4, CV(%): 6.8
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Roux, Schaefers, Clark, Weatherholt, Renaud, Scott, LiPuma, Priebe, Gerard, Yoder-Himes: "A putative lateral flagella of the cystic fibrosis pathogen Burkholderia dolosa regulates swimming motility and host cytokine production." in: PLoS ONE, Vol. 13, Issue 1, pp. e0189810, (2018) (PubMed).

Roux, Weatherholt, Clark, Gadjeva, Renaud, Scott, Skurnik, Priebe, Pier, Gerard, Yoder-Himes: "Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa." in: Infection and immunity, Vol. 85, Issue 6, (2017) (PubMed).
抗原 See all CXCL2 ELISA试剂盒

CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

别名

CXCL2

背景

Protein Function: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein(MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein(MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water(aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.
Synonyms: C-X-C motif chemokine 2,Macrophage inflammatory protein 2,MIP2,Cxcl2,Mip-2, Mip2, Scyb2,
Full Gene Name: C-X-C motif chemokine 2
Cellular Localisation: Secreted.

基因ID

20310

UniProt

P10889

途径

Cellular Response to Molecule of Bacterial Origin