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CXCL2 ELISA 试剂盒

Name: CXCL2 ELISA 试剂盒
SKU: ABIN2859287
Availability: OutOfStock

This Colorimetric ELISA kit is designed for the quantitative measurement of 大鼠 CXCL2. There are 2 publications available.

ELISA

2 images

(2 references)

产品编号 ABIN2859287

发货至: 中国

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Quick Overview for CXCL2 ELISA 试剂盒 (ABIN2859287)

抗原

See all CXCL2 ELISA试剂盒

CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

抗原表位

AA 32-100

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Discover our top product CXCL2 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich Rat MIP-2 ELISA Kit to quantitate Rat Cxcl2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: S32-N100
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 10 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 小鼠 AA 28-100 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 小鼠 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit
CXCL2 适用: 人 Colorimetric Sandwich ELISA 8-1000 pg/mL Cell Lysate, Plasma, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 7.7%, 4.6%, 4%
Inter-Assay Precision (CV%): 9.3%, 4.7%, 4.8%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Zhu, He, Liu, Zhang, Yang, Liu, Wang: "Alpha 1-antitrypsin ameliorates ventilator-induced lung injury in rats by inhibiting inflammatory responses and apoptosis." in: Experimental biology and medicine (Maywood, N.J.), Vol. 243, Issue 1, pp. 87-95, (2018) (PubMed).

Mei, Wang, Li, Liu, Lu, Li, Zhang, Tian: "Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 216, (2017) (PubMed).
抗原 See all CXCL2 ELISA试剂盒

CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

别名

CXCL2

背景

Background: Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.
Gene Full Name: C-X-C motif chemokine ligand 2

基因ID

114105

UniProt

P30348

途径

Cellular Response to Molecule of Bacterial Origin