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GDNF ELISA 试剂盒

Name: GDNF ELISA 试剂盒
SKU: ABIN921058
Availability: OutOfStock

人 GDNF ELISA Kit, Colorimetric is an assay for quantification of 人 GDNF, that has been mentioned in 7+ publications.

ELISA

1 image

(7 references)

产品编号 ABIN921058

发货至: 中国

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Quick Overview for GDNF ELISA 试剂盒 (ABIN921058)

抗原

See all GDNF ELISA试剂盒

GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

抗原表位

AA 109-211

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Discover our top product GDNF ELISA Kit

最低检测浓度

31.2 pg/mL

原理

Sandwich Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit to quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method

Quantitative

特异性

Expression system for standard: NS0
Immunogen sequence: R109-I211
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 4 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 小鼠 Colorimetric Sandwich ELISA 0.9 pg/mL - 60 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 大鼠 AA 78-211 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 小鼠 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 大鼠 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 人 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
GDNF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
Small Sample GDNF 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 7.2%, 6.5%, 4.3%
Inter-Assay Precision (CV%): 9.1%, 6.8%, 4.6%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Niu, Li, Wu, Wu, Yan, Shang, Bai, Li, Hua: "Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells." in: Oncotarget, Vol. 7, Issue 47, pp. 77532-77542, (2018) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Liu, Wang, Shao, Liu: "Genetically modified Schwann cells producing glial cell line-derived neurotrophic factor inhibit neuronal apoptosis in rat spinal cord injury." in: Molecular medicine reports, Vol. 9, Issue 4, pp. 1305-12, (2014) (PubMed).

Banerjee, Nürnberger, Hennerbichler, Riedl, Schuh, Hacobian, Teuschl, Eibl, Redl, Wolbank: "In toto differentiation of human amniotic membrane towards the Schwann cell lineage." in: Cell and tissue banking, Vol. 15, Issue 2, pp. 227-39, (2014) (PubMed).

Chai, Guo, Li, Wang, Wang, Shi, Hu, Liu, Adah: "Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes." in: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 100-7, (2013) (PubMed).

Wang, Hou, Yuan, Hou, Liu, Li, Zhang: "Association study between plasma GDNF and cognitive function in late-onset depression." in: Journal of affective disorders, Vol. 132, Issue 3, pp. 418-21, (2011) (PubMed).

Yang, Zhou, Gao, Chen, Tu, Sun, Liu, He, Liu, Yuan: "Neuroprotective effects of bone marrow stem cells overexpressing glial cell line-derived neurotrophic factor on rats with intracerebral hemorrhage and neurons exposed to hypoxia/reoxygenation." in: Neurosurgery, Vol. 68, Issue 3, pp. 691-704, (2011) (PubMed).
抗原 See all GDNF ELISA试剂盒

GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

别名

GDNF

背景

Background: Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.
Gene Full Name: glial cell derived neurotrophic factor

基因ID

2668

UniProt

P39905

途径

RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration