IL12 ELISA 试剂盒
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Quick Overview for IL12 ELISA 试剂盒 (ABIN8126256)
抗原
See all IL12 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.625 pg/mL
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原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- 9.375 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Canine IL-12/p70 concentrations in serum, plasma. Detection Range: 15.625 - 1000 pg/mL Sensitivity: < 9.375 pg/mL
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Interleukin 12 (IL12) ELISA Kit
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Interleukin 12 (IL12) ELISA KitIL12 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 适用: 绵羊 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-canine IL-12/p70 antibody was pre-coated into 96-well plates. Biotin conjugated anti-canine IL-12/p70 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the canine IL-12/p70 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Canine IL-12/p70 can be calculated using the standard curve.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- IL12 (Interleukin 12 (IL12))
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别名
- IL-12/p70 (Interleukin 12/p70)
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途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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