p21 ELISA 试剂盒
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北京 101111
Quick Overview for p21 ELISA 试剂盒 (ABIN8077764)
抗原
See all p21 (CDKN1A) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- Human Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit for detecting CDKN1A in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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灵敏度
- 0.056 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA Kit
CDKN1A 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验精密度
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 12 months
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- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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别名
- Cyclin Dependent Kinase Inhibitor 1A
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背景
- Gene Aliases: WAF1, p21, CIP1, p21WAF1, CAP20, CDKN1, MDA-6, SDI1, p21CIP1, CDK-Interacting Protein 1, Melanoma differentiation-associated protein 6
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基因ID
- 1026
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UniProt
- P38936
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途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
抗原 See all p21 (CDKN1A) ELISA试剂盒
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