VEGFR2/CD309 ELISA 试剂盒
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Quick Overview for VEGFR2/CD309 ELISA 试剂盒 (ABIN8071280)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Phospho-VEGFR2 (Y1175) and Total VEGFR2 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human cell lysates.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes human vascular endothelial growth factor receptor 2 phosphorylated at site Tyrosine-1175.
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组件
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- Pre-Coated 96-well Strip Microplate
- Positive Control
- Anti-Phospho Detection Antibody
- Anti-Pan Detection Antibody (*not included in phospho-only version)
- HRP-Conjugated Secondary Antibody
- HRP-Streptavidin (*not included in phospho-only version)
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Shaker
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare the positive control or sample dilutions
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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别名
- VEGFR2
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背景
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Gene Symbols: KDR, FLK1, VEGFR2
Protein Name (Synonyms): Vascular endothelial growth factor receptor 2 (VEGFR-2), Fetal liver kinase 1 (FLK-1), Kinase insert domain receptor (KDR), Protein-tyrosine kinase receptor flk-1
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基因ID
- 3791
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UniProt
- P35968
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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