VEGFR2/CD309 ELISA 试剂盒
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北京 101111
Quick Overview for VEGFR2/CD309 ELISA 试剂盒 (ABIN1672753)
抗原
See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit to quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A20-E764Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 4 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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VEGF Receptor 2 (VEGFR2) ELISA Kit
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VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 小鼠 AA 20-762 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 人 Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma, Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 人 Colorimetric Sandwich ELISA 62.50-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
VEGF Receptor 2 (VEGFR2) ELISA KitVEGFR2 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.1%, 6.5%, 7.8%
Inter-Assay Precision (CV%): 6.9%, 7.2%, 8.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation." in: Oncotarget, Vol. 8, Issue 12, pp. 19968-19979, (2017) (PubMed).
: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." in: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).
: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, (2013) (PubMed).
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- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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别名
- KDR
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背景
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Background: Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
Gene Full Name: kinase insert domain receptor
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基因ID
- 3791
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UniProt
- P35968
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒
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