TEK ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for TEK ELISA 试剂盒 (ABIN8070997)
抗原
See all TEK ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Phospho-Tie2 (Tyr992) ELISA Kit measures phosphorylated Tie2 (Tyr992) and total (pan) Tie2 in cell and tissue lysates.
-
Analytical Method
- Semi-Quantitative
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
-
试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
-
-
TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kit
TEK 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 AA 23-745 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 AA 23-744 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
-
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
-
别名
- Tie-2
-
背景
-
Gene Symbols: TEK TIE2 VMCM VMCM1
Protein Name (Synonyms): Angiopoietin-1 receptor (EC 2.7.10.1) (Endothelial tyrosine kinase) (Tunica interna endothelial cell kinase) (Tyrosine kinase with Ig and EGF homology domains-2) (Tyrosine-protein kinase receptor TEK) (Tyrosine-protein kinase receptor TIE-2) (hTIE2) (p140 TEK) (CD antigen CD202b)
-
基因ID
- 7010
-
UniProt
- Q02763
-
途径
- RTK signaling, Growth Factor Binding
抗原 See all TEK ELISA试剂盒
-
