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Quick Overview for TEK ELISA 试剂盒 (ABIN6720115)
抗原
See all TEK ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0311
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Supplier
- Rockland
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原理
- Human TIE2 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A23-K745 -
交叉反应 （详细）
- There is cross-reactivity with human TIE1< 0.1 % .
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kit
TEK 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 AA 23-745 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 小鼠 AA 23-744 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIE2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TIE2 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.8%, 4.4%, 5.6%
Inter-Assay Precision (CV%): 8.7%, 5.8%, 7.1% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
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别名
- TIE2
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背景
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Background: Tyrosine kinase with Ig and EGF homology domain 2 (Tie-2), also called TEK tyrosine kinase, endothelial (TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
Gene Full Name: TEK receptor tyrosine kinase
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基因ID
- 7010
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NCBI登录号
- NP_000450
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UniProt
- Q02763
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途径
- RTK signaling, Growth Factor Binding
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