GCK ELISA 试剂盒
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Quick Overview for GCK ELISA 试剂盒 (ABIN8068914)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Measures nitrated Glucokinase (GCK) and total Glucokinase in human cell and tissue lysates, enabling precise assessment of Glucokinase nitration by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
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Analytical Method
- Quantitative
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组件
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- Size / Qty
- Microplate Coated With Anti-Pan-Glucokinase Antibody
- Anti-Nitration Detection Antibody
- Anti-Glucokinase Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
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Glucokinase (Hexokinase 4) (GCK) ELISA Kit
GCK 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 犬 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- GCK (Glucokinase (Hexokinase 4) (GCK))
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别名
- Glucokinase
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背景
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Gene Symbols: GCK
Background: The Nitrated-Glucokinase and Total-Glucokinase ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human nitrated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan Glucokinase antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Glucokinase present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-nitration antibody is used to detect nitrated Glucokinase or biotinylated anti-Glucokinase antibody is used to detect total Glucokinase . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of nitrated Glucokinase or total Glucokinase bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Hexokinase-4 (HK4), Glucokinase, Hexokinase type IV (HK IV), Hexokinase-D
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基因ID
- 2645
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UniProt
- P35557
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途径
- MAPK Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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