Glucokinase (GCK) Activity Colorimetric Assay Kit
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北京 101111
Quick Overview for Glucokinase (GCK) Activity Colorimetric Assay Kit (ABIN7850002)
抗原
See all GCK 试剂盒检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 2.62 U/L - 42.8 U/L
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最低检测浓度
- 2.62 U/L
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原理
- Glucokinase (GCK) Activity Colorimetric Assay Kit
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产品特性
- Glucokinase (GCK) is a key enzyme in the glucose metabolism pathway. It participates in the first step of glycolysis, catalyzes glucose phosphorylation, promotes insulin secretion and glucose metabolism, and effectively controls blood glucose balance in internal. GCK, also known as hexokinase IV, is mainly expressed in hepatocytes and islet β-cell, and has a stronger affinity for glucose than other hexokinases. Therefore, the total hexokinase activity in the sample is measured at high glucose concentration, and the hexokinase activity with low glucose affinity is measured at low glucose concentration. The GCK activity in the sample can be obtained by subtracting the total hexokinase from the total hexokinase. The detection principle of this kit: GCK catalyzes the substrate reaction to produce NADPH, which reacts with the chromogenic agent. The activity of GCK can be calculated by measuring the generation rate of chromogenic substance at 450 nm.
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Glucokinase (Hexokinase 4) (GCK) ELISA Kit
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Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 大鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Glucokinase (Hexokinase 4) (GCK) ELISA KitGCK 适用: 犬 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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实验时间
- 0.5 h
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实验精密度
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Inter-assay precision: 1.3-7.4%
Intra-assay precision: 2.9-6.6%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- -20 °C
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储存方法
- This product can be stored at -20°C for 12 months with shading light.
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有效期
- 12 months
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- GCK (Glucokinase (Hexokinase 4) (GCK))
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别名
- Glucokinase
抗原
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