CD40 ELISA 试剂盒
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Quick Overview for CD40 ELISA 试剂盒 (ABIN8068302)
抗原
See all CD40 ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
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原理
- Measures nitrated CD40 (TNFRSF5) and total CD40 in human cell and tissue lysates, enabling precise assessment of CD40 nitration by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
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Analytical Method
- Quantitative
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组件
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- Size / Qty
- Microplate Coated With Anti-Pan-CD40 Antibody
- Anti-Nitration Detection Antibody
- Anti-CD40 Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
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CD40 (CD40) ELISA Kit
CD40 适用: 人 Colorimetric Sandwich ELISA 19.531 pg/mL - 1250 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 小鼠 AA 24-193 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD40 (CD40) ELISA KitCD40 适用: 人 AA 21-193 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
CD40 (CD40) ELISA KitCD40 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- CD40
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别名
- CD40
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背景
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Gene Symbols: CD40, TNFRSF5
Background: The Nitrated-CD40 and Total-CD40 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human nitrated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan CD40 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-nitration antibody is used to detect nitrated CD40 or biotinylated anti-CD40 antibody is used to detect total CD40 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of nitrated CD40 or total CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
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基因ID
- 958
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UniProt
- P25942
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途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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