DPP4 ELISA 试剂盒

• Precoated Micro ELISA Plate, Dismountable
• Reference Standard
• Concentrated HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• HRP Conjugate Diluent
• Concentrated Wash Buffer (25x)
• Substrate Reagent
• Stop Solution
• Plate Sealer
• Manual

1. Add 50 µL standard or sample to each well.
2. Add 50 µL HRP Conjugate. Incubate for 60 min at 37 °C.
3. Aspirate and wash 5 times.
4. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
5. Add 50 µL Stop Solution. Read at 450 nm immediately.
6. Calculation of results.

1. Bring all reagents to room temperature (18 - 25 °C) before use.
2. Standard Working Solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard & Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution, then make serial dilutions as needed. Note: Serially diluted standards should be prepared immediately, prior to use.
3. HRP Conjugate Working Solution: Calculate the required amount before the experiment. When preparing, prepare slightly more than calculated. Centrifuge the Concentrated HRP Conjugate at 800×g for 1 min, then dilute the 100× Concentrated HRP Conjugate to 1× working solution with HRP Conjugate Diluent. Note: Prepare the working solution immediately, prior to use.
4. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved.

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level of the target were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level of the target were tested on 3 different plates, 20 replicates in each plate.

Both intra-CV and inter-CV are < 10%.

• For unopened kit: All reagents should be stored according to the labels on the vials. Protect Concentrated HRP Conjugate and Substrate Reagent from light. Always use the reconstituted standard within 24h. Reconstituted standard may be stored aliquoted at -20 °C for up to two weeks. Store samples at – 80 °C for up to 3 months. Avoid repeated freeze-thaw cycles.
• For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Target Synonym: CD26,DPPIV,ADABP,ADCP2,TP103,Adenosine Deaminase Complexing Protein 2

Background: Dipeptidyl Peptidase IV (DPPIV, also CD26 and adenosine deaminase complexing protein 2) is a serine protease that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. As a type II membrane protein, it includes a small cytoplasmic ta