DPP4 ELISA 试剂盒
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Quick Overview for DPP4 ELISA 试剂盒 (ABIN1672773)
抗原
See all DPP4 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich Human CD26/DPP4 ELISA Kit to quantitate Human DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: D34-P766Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Dipeptidyl-Peptidase 4 (DPP4) ELISA Kit
DPP4 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.5%, 6.4%, 6.2%
Inter-Assay Precision (CV%): 4.9%, 8.3%, 7.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Serum CD26 levels in patients with gastric cancer: a novel potential diagnostic marker." in: BMC cancer, Vol. 15, pp. 703, (2016) (PubMed).
: "Extra virgin olive oil use is associated with improved post-prandial blood glucose and LDL cholesterol in healthy subjects." in: Nutrition & diabetes, Vol. 5, pp. e172, (2015) (PubMed).
: "Increase in the number of presynaptic large intramembrane particles during synaptic transmission at the Torpedo nerve-electroplaque junction." in: Neuroscience, Vol. 19, Issue 1, pp. 63-79, (1986) (PubMed).
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- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
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别名
- DPP4
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背景
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Background: Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
Gene Full Name: dipeptidyl peptidase 4
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基因ID
- 1803
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UniProt
- P27487
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途径
- Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
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