DPP4 ELISA 试剂盒
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Quick Overview for DPP4 ELISA 试剂盒 (ABIN6720242)
抗原
See all DPP4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0402
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Supplier
- Rockland
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原理
- Mouse CD26 - DPP4 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S29-H760 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Dipeptidyl-Peptidase 4 (DPP4) ELISA Kit
DPP4 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitQuick DPP4 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Biological Fluids, Plasma, Serum
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 人 AA 34-766 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Dipeptidyl-Peptidase 4 (DPP4) ELISA KitDPP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD26 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD26 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 8.7%, 7.5%, 5.6%
Inter-Assay Precision (CV%): 6.7%, 8.7%, 6.2% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
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别名
- CD26/DPP4
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背景
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Background: Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
Gene Full Name: dipeptidylpeptidase 4
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基因ID
- 13482
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NCBI登录号
- NP_001153015
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UniProt
- P28843
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途径
- Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
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