TGFA ELISA 试剂盒
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Quick Overview for TGFA ELISA 试剂盒 (ABIN7830912)
抗原
See all TGFA ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- TGFA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TGFA expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitSmall Sample TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 人 AA 40-89 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 马 AA 40-89 Colorimetric Sandwich ELISA
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 兔 AA 40-89 Colorimetric Sandwich ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGFA
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背景
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Synonyms: TFGA
Gene Description: transforming growth factor, alpha
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基因ID
- 7039
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原 See all TGFA ELISA试剂盒
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