SHP1 ELISA 试剂盒
The 人 SHP1 ELISA Kit (ABIN7830867) is a Colorimetric ELISA Kit designed to quantify 人 SHP1.
产品编号 ABIN7830867
发货至:
中国
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Quick Overview for SHP1 ELISA 试剂盒 (ABIN7830867)
抗原
See all SHP1 (PTPN6) ELISA试剂盒
SHP1 (PTPN6)
(Protein-tyrosine Phosphatase 1C (PTPN6))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- PTPN6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PTPN6 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Protein-tyrosine Phosphatase 1C (PTPN6) ELISA Kit
PTPN6 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
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别名
- PTPN6
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背景
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Synonyms: HCP|HCPH|HPTP1C|PTP-1C|SH-PTP1|SHP-1|SHP-1L|SHP1
Gene Description: protein tyrosine phosphatase, non-receptor type 6
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基因ID
- 5777
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途径
- JAK/STAT Signaling, TCR Signaling, TLR signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling
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